Difference between revisions of "Part:BBa K361001"

Latest revision as of 03:28, 7 November 2010

Message gene template

This message system consists of a message sequence, with specific repeat sequences (repeat A: repeat 1 and 2 as a pair) inserted as to be recognized by the Rci site-specific recombinase.

This message has 70 characters - "We must learn to live together as brothers or perish together as tools"
In a 4 bits system, the total sequence is 280 bp.

In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.

"Fig.1 - The structure of our message"

Reference:

[1] Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 10
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 404
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 7
Illegal BamHI site found at 427
Illegal XhoI site found at 433
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 10
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 10
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K361001 short</partinfo>
-This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.
+This message system consists of a message sequence, with specific repeat sequences (repeat A: repeat 1 and 2 as a pair) inserted as to be recognized by the Rci site-specific recombinase.<br>
+*This message has 70 characters - "We must learn to live together as brothers or perish together as tools"<br>
+*In a 4 bits system, the total sequence is 280 bp.<br>
-*This message has 70 characters:
-We must learn to live together as brothers or perish together as tools
-*In a 4 bits system, the total sequence is 280 bp.
 In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.
-[[Image:msg.png]]
+[[Image:Our_message_structure.png|thumb|"Fig.1 - The structure of our message"]]
 *Reference:
-Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.
+[1] Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.
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