Difference between revisions of "Part:BBa K313006"

Latest revision as of 02:49, 28 October 2010

MS2 phage cDNA (XbaI site is removed)

This part is a cDNA of the MS2 phage genome. (XbaI site is removed.)

We succeeded in making this cDNA from the original RNA of the phage by RT-PCR.

This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift.

The sequence of the MS2 phage genome originally includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
Illegal BamHI site found at 2057
Illegal XhoI site found at 1048
Illegal XhoI site found at 3214
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 103
Illegal EcoRI site found at 1628
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 1733
Illegal NgoMIV site found at 2437
Illegal NgoMIV site found at 3309
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1665
Illegal BsaI.rc site found at 163
Illegal BsaI.rc site found at 2257

@@ Line 9: / Line 9: @@
 The sequence of the MS2 phage genome originally includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis.
+Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2  Phage MS2] assay page.
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