Difference between revisions of "Part:BBa K395600"
 
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We designed apple fragrance expression device with MpAAT1<sup>[[media:リンゴのにおい.pdf|[1]]]</sup>. Fig. 1 shows the outline of the device. MpAAT1 converts substrate and Acetyl CoA into apple fragrance molecules.　Acetyl CoA exists originally in E.coli cell. MpAAT1 converts 2-methyl butanol and butanol into 2-methylbutyl acetate and butyl acetate respectively.
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We designed apple fragrance expression device with MpAAT1<sup>[1]</sup>. Fig. 1 shows the outline of the device. MpAAT1 converts substrate and Acetyl CoA into apple fragrance molecules.　Acetyl CoA exists originally in E.coli cell. MpAAT1 converts 2-methyl butanol and butanol into 2-methylbutyl acetate and butyl acetate respectively.
  
  
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We performed gas chromatography to confirm the production of the esters, and the results revealed that MpAAT1 successfully converted alcohol and Acetyl CoA into our target esters (Fig. 2).
 
We performed gas chromatography to confirm the production of the esters, and the results revealed that MpAAT1 successfully converted alcohol and Acetyl CoA into our target esters (Fig. 2).
  
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[[Image:GC result.png|600px|center|thumb|Fig. 2.  Gas chromatography analysis of apple fragrance expression device.<br>(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.<Br>
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[[Image:Tokyotech gas chromatograpy.png|600px|center|thumb|Fig. 2.  Gas chromatography analysis of apple fragrance expression device.<br>(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.<Br>
 
This work is done by Toshitaka Matsubara.]]
 
This work is done by Toshitaka Matsubara.]]
  

Latest revision as of 02:43, 9 November 2010

Apple fragrance enzyme (MpAAT1) generator

BBa_K395600 produce MpAAT1 enzyme that catalyzes production of 2-methylbutyl acetate and butyl acetate from 2-methylbutanol and butanol. 2-methylbutyl acetate and butyl acetate has a apple fragrance.


We designed apple fragrance expression device with MpAAT1[1]. Fig. 1 shows the outline of the device. MpAAT1 converts substrate and Acetyl CoA into apple fragrance molecules.　Acetyl CoA exists originally in E.coli cell. MpAAT1 converts 2-methyl butanol and butanol into 2-methylbutyl acetate and butyl acetate respectively.


Fig. 1. Apple fragrance expression device.


We performed gas chromatography to confirm the production of the esters, and the results revealed that MpAAT1 successfully converted alcohol and Acetyl CoA into our target esters (Fig. 2).

Fig. 2. Gas chromatography analysis of apple fragrance expression device.
(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.
This work is done by Toshitaka Matsubara.

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter2 more information])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 195
    Illegal BamHI site found at 1635
    Illegal XhoI site found at 1675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 205
    Illegal AgeI site found at 1419
  • 1000
    COMPATIBLE WITH RFC[1000]