Difference between revisions of "Part:BBa K316024"

Latest revision as of 23:56, 27 October 2010

Chloraphenicol resistance gene with dif and 5amyE

This part is identical to BBa_K143023 except for the dif siteBBa_K3160 integrated behind the 5' amye BBa_K143001 integration sequence.

Chloramphenicol acetyltransferase (CAT) BBa_J31005 confers resistance to chloramphenicol, a common lab selection antibiotic. The 5' amyE BBa_K143001 integration sequence allows integration into B. subtilis genome, which disrupts ability to breakdown starch. In order for integration to occur, 3' amyE integration sequence BBa_K143002 is also required.

This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce BBa_K143002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 529
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce <bbpart>BBa_K143002</bbpart>.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-[[Image:VectorAMYE5d.PNG|center|800px]]
+[[Image:VectorAMYE5d.PNG|center|500px]]
+<!-- Add more about the biology of this part here
+===Usage and Biology===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 <partinfo>BBa_K316024 SequenceAndFeatures</partinfo>