Difference between revisions of "Part:BBa K322210"
 
(One intermediate revision by the same user not shown)
Line 6: Line 6:
 
Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions.
 
Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions.
  
−
Notes: This biobrick was submitted in compliance with RFC54, which was jointly developed by Osaka and us. It has complete suffix but prefix lacks NotI site. It is still completely compatible with RFC10.  
+
Notes: This biobrick has complete suffix but prefix lacks NotI site, in compliance with RFC54. It is still completely compatible with RFC10 assembly.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:22, 27 October 2010

Chloramphenicol acetyltransferase (cat)

cat is an antibiotic resistance gene, used in the first step of BRIDGE which requires the deletion of existing DNA (probably a non-coding piece or a non-essential gene) to select for cells which have taken up the construct by growing them on the relevant antibiotic.

Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions.

Notes: This biobrick has complete suffix but prefix lacks NotI site, in compliance with RFC54. It is still completely compatible with RFC10 assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]