Difference between revisions of "Part:BBa K320005"

 
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This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>).
 
This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>).
  
This BioBrick was principally built to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>).  
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This BioBrick was built to test the constitutive promoter (<partinfo>BBa_K320002</partinfo>).  
  
We only manage to characterize it in E.Coli (see the [https://parts.igem.org/Part:BBa_K320005:Experience Experience]). Further, we will characterize it in Asaia to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''new chassis''].
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It has only been characterized in ''E. coli'' (see the [https://parts.igem.org/Part:BBa_K320005:Experience Experience]). We are currently running experiments to characterize it in ''Asaia'' to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis].
  
 
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Latest revision as of 18:21, 27 October 2010

Constitutive expression of CFP

This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).

This BioBrick was built to test the constitutive promoter (BBa_K320002).

It has only been characterized in E. coli (see the Experience). We are currently running experiments to characterize it in Asaia to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
    Illegal NheI site found at 146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]