Difference between revisions of "Part:BBa K320005"

Latest revision as of 18:21, 27 October 2010

Constitutive expression of CFP

This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).

This BioBrick was built to test the constitutive promoter (BBa_K320002).

It has only been characterized in E. coli (see the Experience). We are currently running experiments to characterize it in Asaia to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 62
Illegal NheI site found at 146
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>).
-This BioBrick was principally built to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>).
+This BioBrick was built to test the constitutive promoter (<partinfo>BBa_K320002</partinfo>).
-We only manage to characterize it in E.Coli (see the [https://parts.igem.org/Part:BBa_K320005:Experience Experience]). Further, we will characterize it in Asaia to continue building gear for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia''].
+It has only been characterized in ''E. coli'' (see the [https://parts.igem.org/Part:BBa_K320005:Experience Experience]). We are currently running experiments to characterize it in ''Asaia'' to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis].
 <!-- Add more about the biology of this part here