Difference between revisions of "Part:BBa K320004:Design"

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===Design Notes===
 
===Design Notes===
−
We ligate BioBricks <partinfo>BBa_K320000</partinfo> and <partinfo>BBa_P1003</partinfo>  
+
The first subpart (<partinfo>BBa_K320000</partinfo>) is taken from the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzymes SpeI and PstI to take our part out.
  
−
The first BioBrick (<partinfo>BBa_K320000</partinfo>) was intial in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzyme SpeI and PstI.
+
The second subpart (<partinfo>BBa_p1003</partinfo>) was also in the <partinfo>pSB1C3</partinfo> backbone vector. To extract it, we digested with Xba and PstI.
−
 
+
−
The second BioBrick (<partinfo>BBa_p1003</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. This one we digested with Xba and PstI.
+
  
 
===Source===
 
===Source===
−
The BioBricks assembled from <partinfo>BBa_K320000</partinfo> and <partinfo>BBa_P1003</partinfo>.
+
The BioBricks were assembled from <partinfo>BBa_K320000</partinfo> and <partinfo>BBa_P1003</partinfo>.
  
 
===References===
 
===References===

Latest revision as of 18:15, 27 October 2010

Origin for Asaia and resistance to Kanamycin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


Design Notes

The first subpart (BBa_K320000) is taken from the pSB1C3 backbone vector. We opened it with the restriction enzymes SpeI and PstI to take our part out.

The second subpart (BBa_P1003) was also in the pSB1C3 backbone vector. To extract it, we digested with Xba and PstI.

Source

The BioBricks were assembled from BBa_K320000 and BBa_P1003.

References