Difference between revisions of "Part:BBa K320001:Design"

(→‎Design Notes)
(Undo revision 96704 by Gwen (Talk))
 
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===Design Notes===
 
===Design Notes===
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The DNA was optimized for synthesis in ''E. coli'' using the Mr. Gene programm. The sequence should also work better in ''Asaia''.
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The DNA was optimized for synthesis in ''E. coli'' and Asaia.
  
 
===Source===
 
===Source===

Latest revision as of 15:57, 27 October 2010

Immunotoxin against P.Berghei


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 957
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472
    Illegal BsaI site found at 699


Design Notes

The DNA was optimized for synthesis in E. coli and Asaia.

Source

Coding sequence comes from [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X [1]].

References

  1. [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]