Difference between revisions of "Part:BBa K320006:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We | + | We ligated the two BioBricks quoted below. |
− | + | The first BioBrick (<partinfo>BBa_K320001</partinfo>) was initially in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzymes SpeI and PstI. | |
+ | |||
+ | The second part (<partinfo>BBa-K320002</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. We digested this one with Xba and PstI. | ||
===Source=== | ===Source=== | ||
− | The Two BioBricks we used are BBa_K320001 and | + | The Two BioBricks we used are <partinfo>BBa_K320001</partinfo> and <partinfo>BBa_K320002</partinfo>. |
===References=== | ===References=== | ||
+ | # [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes] |
Latest revision as of 16:07, 27 October 2010
Constitutive expression of Immunotoxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 883
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ligated the two BioBricks quoted below.
The first BioBrick (BBa_K320001) was initially in the pSB1C3 backbone vector. We opened it with the restriction enzymes SpeI and PstI.
The second part (BBa_K320002) was also stored in the pSB1C3 backbone vector. We digested this one with Xba and PstI.
Source
The Two BioBricks we used are BBa_K320001 and BBa_K320002.
References
- [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]