Difference between revisions of "Part:BBa K348000:Design"
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===Design Notes=== | ===Design Notes=== | ||
It was amplified by PCR using those primers : | It was amplified by PCR using those primers : | ||
| + | |||
CCCGAATTCGCGGCCGCTTCTAGATGAAGGTAGATATT | CCCGAATTCGCGGCCGCTTCTAGATGAAGGTAGATATT | ||
CCCCTGCAGCGGCCGCTACTAGTATCAGAGCGCCTGGGT | CCCCTGCAGCGGCCGCTACTAGTATCAGAGCGCCTGGGT | ||
| − | + | No special safety issues exist surrounding this part. | |
===Source=== | ===Source=== | ||
Latest revision as of 15:28, 27 October 2010
TdCE - pyruvate formate lyase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1107
Illegal AgeI site found at 511
Illegal AgeI site found at 1861 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was amplified by PCR using those primers :
CCCGAATTCGCGGCCGCTTCTAGATGAAGGTAGATATT
CCCCTGCAGCGGCCGCTACTAGTATCAGAGCGCCTGGGT
No special safety issues exist surrounding this part.
Source
It comes from the genome of Escherichia coli K-12 substr. MG1655
References
[http://ecoliwiki.net/colipedia/index.php/ECK3103] [http://biocyc.org/ecoli/new-image?object=G7627]