Difference between revisions of "Part:BBa K376015:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K376015=== | ===Applications of BBa_K376015=== | ||
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| + | This Lux promoter tester was used in an experiment to measure the effectiveness of AHL as an inducer in Lux promoters. The LuxR was under constitutive expression so that the limiting factor would be AHL. The coding sequences for LuxR and RFP were preceded by the standard RBS B0034. | ||
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| + | The AHL used was OC6-homoserine lactone. It was added to samples in a 96-well plate in concentrations of 0, .1, 1, 10, 100 and 1000nM. | ||
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| + | The results [http://2010.igem.org/Team:Penn_State/Project#Lux_Promoter_Characterization on our wiki] show no trend in protein expression based on concentration of OC6-homoserine lactone. More research is warranted to provide information about what are the best conditions for chemically inducing the Lux promoters. | ||
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| + | [[image:1107_116_tetR.png|800px]] | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 18:21, 7 November 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K376015
This Lux promoter tester was used in an experiment to measure the effectiveness of AHL as an inducer in Lux promoters. The LuxR was under constitutive expression so that the limiting factor would be AHL. The coding sequences for LuxR and RFP were preceded by the standard RBS B0034.
The AHL used was OC6-homoserine lactone. It was added to samples in a 96-well plate in concentrations of 0, .1, 1, 10, 100 and 1000nM.
The results [http://2010.igem.org/Team:Penn_State/Project#Lux_Promoter_Characterization on our wiki] show no trend in protein expression based on concentration of OC6-homoserine lactone. More research is warranted to provide information about what are the best conditions for chemically inducing the Lux promoters.
User Reviews
UNIQebd380f16f4bc243-partinfo-00000000-QINU UNIQebd380f16f4bc243-partinfo-00000001-QINU