Difference between revisions of "Part:BBa K399002:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K399002 short</partinfo>
 
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===Source===
 
===Source===
  
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The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University.
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mTangerine was developed by Roger Tsien<sup>1</sup>.
  
 
===References===
 
===References===
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1.  Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572

Latest revision as of 03:40, 28 October 2010

mTangerine


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mTangerine contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and SpeI.


Source

mTangerine was developed by Roger Tsien1.

References

1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572