Difference between revisions of "Part:BBa K395705"
 
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This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.
 
This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.
  
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[[Image:tokyotech_registry-metabolite705.jpg|left|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
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[[Image:Tokyotech registry-asta.jpg|left|700px|thumb|Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC<br>This work is done by Yumiko Kinoshita]]
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[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
 
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[[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|460px|Fig. 2-1-12-2 astaxanthin extract]]
 
  
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[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
 
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[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|400px|Fig. 2-1-1-3.TLC.jpg]]
 
  
  

Latest revision as of 00:11, 28 October 2010

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.

Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC
This work is done by Yumiko Kinoshita





















In order to confirm that BBa_K395705 is worked, we construct a BioBrick part (BBa_K395706), which contains this part under BBa_K395702. We synthesized astaxanthin by BBa_K395706, when add beta-carotene as a substrate. Thus we confirmed that BBa_395705 is working. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])






Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715
    Illegal SapI site found at 542
    Illegal SapI site found at 554