Difference between revisions of "Part:BBa K320011"
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<partinfo>BBa_K320011 short</partinfo> | <partinfo>BBa_K320011 short</partinfo> | ||
− | This BioBrick contains an origin of replication for Asaia and ''E. coli'' (<partinfo> | + | This BioBrick contains an origin of replication for Asaia and ''E. coli'' (<partinfo>BBa_K320000</partinfo>) and a resistance to Ampicillin (<partinfo>BBa_P1002</partinfo>). It was initially designed to be inserted into (<partinfo>BBa_I51020</partinfo>). |
Warning: Don't try to use this in Asaia. Asaia is naturally resistant against Ampicillin, see our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia website]. | Warning: Don't try to use this in Asaia. Asaia is naturally resistant against Ampicillin, see our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia website]. | ||
Latest revision as of 12:53, 27 October 2010
Origin for Asaia and resistance to Ampicillin
This BioBrick contains an origin of replication for Asaia and E. coli (BBa_K320000) and a resistance to Ampicillin (BBa_P1002). It was initially designed to be inserted into (BBa_I51020). Warning: Don't try to use this in Asaia. Asaia is naturally resistant against Ampicillin, see our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia website].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1166
Illegal AgeI site found at 1326 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4