Difference between revisions of "Part:BBa K320002:Design"

(→‎Design Notes)
(→‎Source)
 
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The sequence was artificially made from two primers containing the ''E. coli'' consensus sequences for promoter and RBS with an appropriate spacing.
 
The sequence was artificially made from two primers containing the ''E. coli'' consensus sequences for promoter and RBS with an appropriate spacing.
  
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===Source===
 
  
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The source is the article [http://www.pnas.org/content/104/21/9047.long  [1]].
 
  
 
===References===
 
===References===
  
 
# [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]
 
# [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]

Latest revision as of 12:33, 27 October 2010

Strong promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was artificially made from two primers containing the E. coli consensus sequences for promoter and RBS with an appropriate spacing.


References

  1. [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]