Difference between revisions of "Part:BBa K320006:Design"

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===Design Notes===
 
===Design Notes===
−
Ligation of the two parts mentioned below.
+
We ligated the two BioBricks quoted below.
 +
 
 +
The first BioBrick (<partinfo>BBa_K320001</partinfo>) was initially in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzymes SpeI and PstI.
 +
 
 +
The second part (<partinfo>BBa-K320002</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. We digested this one with Xba and PstI.
  
 
===Source===
 
===Source===
−
The Two BioBricks we used are BBa_K320001 and BBa-K320002.
+
The Two BioBricks we used are <partinfo>BBa_K320001</partinfo> and <partinfo>BBa_K320002</partinfo>.
  
 
===References===
 
===References===
 +
# [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]

Latest revision as of 16:07, 27 October 2010

Constitutive expression of Immunotoxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ligated the two BioBricks quoted below.

The first BioBrick (BBa_K320001) was initially in the pSB1C3 backbone vector. We opened it with the restriction enzymes SpeI and PstI.

The second part (BBa_K320002) was also stored in the pSB1C3 backbone vector. We digested this one with Xba and PstI.

Source

The Two BioBricks we used are BBa_K320001 and BBa_K320002.

References

  1. [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]