Difference between revisions of "Part:BBa K313035"
 
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<partinfo>BBa_K313035 short</partinfo>
 
<partinfo>BBa_K313035 short</partinfo>
  
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This is a device that aims to test the recombination ability of Hin DNA invertase.
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This is a device that aims to test the recombination ability of the Hin DNA invertase.
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This part is composed of "T7 promoter-lox71-Tet resistance(reverse)-rbs(reverse)-lox66-double terminator."
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lox71 and lox66 are arranged so that the intermediate Tet resistance(reverse)-rbs(reverse) is inverted when Cre is expressed. So, this unit can be used as a detector of Cre, because cells with this construct will survive in the medium with tetracycline only when the cells express Cre.
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Two hixCs are arranged so that the intermediate double terminator is cut out and the RFP begins to express when Hin is expressed. So, this unit can be used as a detector of Hin, because cells with this construct will look red only when the cells express Hin.
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.<br/>
  
  

Latest revision as of 03:55, 28 October 2010

Hin invertase assay device

This is a device that aims to test the recombination ability of the Hin DNA invertase.

Two hixCs are arranged so that the intermediate double terminator is cut out and the RFP begins to express when Hin is expressed. So, this unit can be used as a detector of Hin, because cells with this construct will look red only when the cells express Hin. Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]