Difference between revisions of "Part:BBa K320005:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_K320005===
 
===Applications of BBa_K320005===
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We used this BioBrick to characterize the strong promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br />
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We used this BioBrick to characterize the promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By monitoring the CFP expression in time we could measure the activity of the promoter. Moreover to see if the presence of the CFP hindrate the growth of the clones we measured the growth curve.<br />
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Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br />
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Both measurements were done contemporaneously in E<i>.coli</i> DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br />
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The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.
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The control for the autofluorescence and the growthcurve were done with a wildtype strain of DH5a.
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Unfortunately we can't compare our measurements with a well known promoter.<br />
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Further characterisation will focus on the measurement of the promoter's activity in Asaia.
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[[Image:https://static.igem.org/mediawiki/2010/thumb/3/39/EPFL_OD_prom.png/800px-EPFL_OD_prom.png|800px|center]]
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[[Image:800px-EPFL_OD_prom.png|800px|center]]
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[[Image:https://static.igem.org/mediawiki/2010/thumb/6/61/EPFL_CFP_prom.png/800px-EPFL_CFP_prom.png|800px|center]]
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[[Image:PromOD.png|800px|center]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 16:37, 27 October 2010

Applications of BBa_K320005

We used this BioBrick to characterize the strong promoter we want to use in Asaia BBa_K320002. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.
Both measurements were done simultaneously in the E. coli strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.


800px-EPFL OD prom.png
PromOD.png

User Reviews

UNIQb411fd30445a5b67-partinfo-00000001-QINU UNIQb411fd30445a5b67-partinfo-00000002-QINU