Difference between revisions of "Part:BBa K337017"
 
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<partinfo>BBa_K337017 parameters</partinfo>
 
<partinfo>BBa_K337017 parameters</partinfo>
 
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=== Characterization of the bidirectional CMV promoter ===
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The bidirectional CMV promoter is a new standard part out of our [http://2010.igem.org/Team:Heidelberg/Project/miMeasure miMeasure construct].
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The promoter was used to express two reporter genes with a comparable transcription rate. Referring to the figures below, this is the case.
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[[Image:HeLa nobinding imageOverview.png|thumb|center|600px| Left panel: EGFP channel. Middle panel: EBFP channel. Right panel: merge of both the others.]]
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[[Image:GFPvsBFPHeLa nobinding miM.png|thumb|center|400px| EBFP intensities against EGFP intensities. Correlation coefficient is given.]]

Latest revision as of 02:06, 28 October 2010

bidirectional CMVmin Promoter

Bidirectional CMV promoter driving the GOI on (+) and/or (-) strand (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization of the bidirectional CMV promoter

The bidirectional CMV promoter is a new standard part out of our [http://2010.igem.org/Team:Heidelberg/Project/miMeasure miMeasure construct]. The promoter was used to express two reporter genes with a comparable transcription rate. Referring to the figures below, this is the case.

Left panel: EGFP channel. Middle panel: EBFP channel. Right panel: merge of both the others.
EBFP intensities against EGFP intensities. Correlation coefficient is given.