Difference between revisions of "Part:BBa C0072:Design"
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===Design Notes===
 
===Design Notes===
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wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.<br/><br/>
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wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.<br>
−
 
+
sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).<br><br>
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sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).<br/><br/>
+
−
 
+
−
Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor <br/>
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−
Kendall, KL; Sauer, RT <br/>
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−
The Journal of Biological Chemistry, 264(23):13706-13710, 1989
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−
 
+
−
 
+
  
 +
Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor<br>
 +
Knight, KL; Sauer, RT<br>
 +
The Journal of Biological Chemistry, 264(23):13706-13710, 1989<br>
 +
(PMID: 2668272)
  
 
===Source===
 
===Source===
  
 
enterobacteriophage p22
 
enterobacteriophage p22
 +
Organism host: Salmonella typhimurium [TaxID: 602] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=602 NCBI],[http://www.ebi.ac.uk/newt/display?from=us&search=602 NEWT])
  
 
===References===
 
===References===
 +
Databases:
 +
*UniProt: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=RMNT_BPP22 RMNT_BPP22; P03049; Q7PCJ4]
 +
*PDB: [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1MNT 1MNT]

Latest revision as of 22:36, 27 July 2006


mnt repressor (strong) from Salmonella phage P22 (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.
sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).

Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor
Knight, KL; Sauer, RT
The Journal of Biological Chemistry, 264(23):13706-13710, 1989
(PMID: 2668272)

Source

enterobacteriophage p22 Organism host: Salmonella typhimurium [TaxID: 602] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?lvl=0&id=602 NCBI],[http://www.ebi.ac.uk/newt/display?from=us&search=602 NEWT])

References

Databases:

  • UniProt: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=RMNT_BPP22 RMNT_BPP22; P03049; Q7PCJ4]
  • PDB: [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1MNT 1MNT]