Difference between revisions of "Part:BBa K313019"
 
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If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
 
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
  
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With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we would be able to detect the quantity of Cre and assess the leakiness of the terminator.
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With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:34, 28 October 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of terminator BBa_B0014

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]