Difference between revisions of "Part:BBa K395146"
 
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[[Image:tokyotech_Fig.K395146-1.jpg|300px|left|thumb|Fig. 1 '''GFP fluorescence'''  we gathered the supernatant of  K395146, divided it into two and induced two sample of reporter cell.]]
 
[[Image:tokyotech_Fig.K395146-1.jpg|300px|left|thumb|Fig. 1 '''GFP fluorescence'''  we gathered the supernatant of  K395146, divided it into two and induced two sample of reporter cell.]]
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[[Image:tokyotech_Fig.K395146-1.jpg|300px|left|thumb|Fig. 2 '''the relationship between GFP fluorescence and the concentration of  3OC6HSL''' ]]
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[[Image:tokyotech_LuxR ractivatio promoter assay2.jpg|300px|left|thumb|Fig. 2 '''the relationship between GFP fluorescence and the concentration of  3OC6HSL''' ]]
  
  
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We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of  3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS.
 
  
  
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We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of  3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/luxI_assay  more information])
  
  

Latest revision as of 21:09, 27 October 2010

constitutive LuxI generator

This is a constitutive LuxI generator using P(Lac)IQ (High Transcription) with RBS and Terminator.

The level of activity of K395146 is equal to about 10 nM 3OC6HSL.


Fig. 1 GFP fluorescence we gathered the supernatant of K395146, divided it into two and induced two sample of reporter cell.
Fig. 2 the relationship between GFP fluorescence and the concentration of 3OC6HSL












We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of 3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/luxI_assay more information])




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]