Difference between revisions of "Part:BBa K395706"
 
(7 intermediate revisions by 2 users not shown)
Line 5: Line 5:
  
 
It is necessary to add either beta-carotene or the synthesis operon.
 
It is necessary to add either beta-carotene or the synthesis operon.
 +
[[Image:Tokyotech registry-asta.jpg|left|700px|thumb|Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC<br>This work is done by Yumiko Kinoshita]]
  
−
[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
 
−
[[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|500px|Fig. 2-1-12-2 astaxanthin extract]]
 
  
−
[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
 
−
[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|400px|Fig. 2-1-1-3.TLC.jpg]]
 
  
  
Line 31: Line 28:
  
  
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
Those work are done by Yumiko Kinoshita.<br>
 
These parts contain ''crtW'' (&beta;-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in ''E. coli'' strain MG1655.  (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
 
These parts contain ''crtW'' (&beta;-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in ''E. coli'' strain MG1655.  (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
  

Latest revision as of 00:15, 28 October 2010

rbs+crtZW under pBad promoter for double plasmids

This part contains crtZW, which works as the astaxanthin biosynthesis pathway when add beta-carotene as a substrate.

It is necessary to add either beta-carotene or the synthesis operon.

Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC
This work is done by Yumiko Kinoshita




























Those work are done by Yumiko Kinoshita.
These parts contain crtW (β-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2405
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2488
    Illegal SapI site found at 961
    Illegal SapI site found at 2315
    Illegal SapI site found at 2327