Difference between revisions of "Part:BBa K337023"
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| − | RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by double Tet operator. | + | RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by double Tet operator. Therefor part allows for the regulation of the GOI via the Tet System (BB-2, RFC12). |
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Latest revision as of 09:14, 27 October 2010
BGH(rc)/shRNA10(rc)/RSV(rc)/CMV(fw)_TetO2(fw)
RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by double Tet operator. Therefor part allows for the regulation of the GOI via the Tet System (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1659
Illegal BamHI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]