Difference between revisions of "Part:BBa K349001:Experience"
(→Applications of BBa_K349001) |
(→Applications of BBa_K349001) |
||
| Line 4: | Line 4: | ||
===Applications of BBa_K349001=== | ===Applications of BBa_K349001=== | ||
| − | + | Digestion with BsaI around the recognition sites inside of the prefix and suffix will create an RFP cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the RFP cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes. | |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 07:23, 6 November 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K349001
Digestion with BsaI around the recognition sites inside of the prefix and suffix will create an RFP cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the RFP cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes.
User Reviews
UNIQ597ca919d8e36e57-partinfo-00000000-QINU UNIQ597ca919d8e36e57-partinfo-00000001-QINU