Difference between revisions of "Part:BBa K395702"

Latest revision as of 23:48, 27 October 2010

rbs+crtZ under pBad promoter for double plasmids

This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.

It is necessary to add either beta-carotene or the synthesis operon.this part applies to ② and ④

Fig.2 zeaxanthin pellet and plate, TLC
These works are done by Yumiko Kinoshita

These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 6: / Line 6: @@
 It is necessary to add either beta-carotene or the synthesis operon.this part applies to ② and ④
-[[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]]
+[[Image:Tokyotech_registry_zeaxanthin.jpg|center|650px|thumb|Fig.2 zeaxanthin pellet and plate, TLC<br>These works are done by Yumiko Kinoshita]]
-[[Image:tokyotech_table. 1.1.1.2.jpg|left|450px|figure1]]
-[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]]
 These parts contain ''crtZ'' (&beta;-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of &beta;-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655.  (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])