Difference between revisions of "Part:BBa K337030"
 
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<partinfo>BBa_K337030 short</partinfo>
 
<partinfo>BBa_K337030 short</partinfo>
  
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Luc2 with XmaI/XhoI site inserted in the 3'UTR right behind the stop codon (BB-2, RFC12). XmaI/XhoI were introduced into the 3'UTR right after the stop codon for enabling the introduction of microRNA binding sites.
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Firefly luciferase Luc2 with XmaI/XhoI cutting sites in the 3'UTR enabling easy introduction of synthetic mircoRNA binding sites  (BB-2, RFC12).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:55, 27 October 2010

Luc2_SV40 (microRNA binding site compatible)

Firefly luciferase Luc2 with XmaI/XhoI cutting sites in the 3'UTR enabling easy introduction of synthetic mircoRNA binding sites (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1944
    Illegal XhoI site found at 1686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 70
    Illegal NgoMIV site found at 1414
    Illegal NgoMIV site found at 1435
    Illegal NgoMIV site found at 1707
    Illegal AgeI site found at 1138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1320