Difference between revisions of "Part:BBa K337027"
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<partinfo>BBa_K337027 short</partinfo> | <partinfo>BBa_K337027 short</partinfo> | ||
| − | CMV Promoter with Tet Operator (TetO2) (BB-2, RFC12). We testet this part by dual luciferase assay in context of Part BBa_K337038 and BBa_K337046 by transfection into Hek-Trex cells, having a CMV driven TetR construct stably integrated. A complete knockdown was observed (value K3), whereas the RSV and CMV showed a stable and high expression. Transfection into other cell lines led to stable high expression, showing, that the part is working. | + | CMV Promoter with Tet Operator (TetO2) (BB-2, RFC12). We testet this part by dual luciferase assay in context of Part BBa_K337038 and BBa_K337046 by transfection into Hek-Trex cells, having a CMV driven TetR construct stably integrated. A complete knockdown was observed (value K3), whereas the RSV and CMV showed a stable and high expression. Transfection into other cell lines led to stable high expression, showing, that the part is working (BB-2, RFC12). |
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Latest revision as of 09:07, 27 October 2010
CMV_TetO2
CMV Promoter with Tet Operator (TetO2) (BB-2, RFC12). We testet this part by dual luciferase assay in context of Part BBa_K337038 and BBa_K337046 by transfection into Hek-Trex cells, having a CMV driven TetR construct stably integrated. A complete knockdown was observed (value K3), whereas the RSV and CMV showed a stable and high expression. Transfection into other cell lines led to stable high expression, showing, that the part is working (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 605
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]