Difference between revisions of "Part:BBa K399001:Design"

 
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<partinfo>BBa_K399001 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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mStrawberry contains a PstI site.  To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI.  Digest the upstream sequence at EcoRI and Spe1.
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mStrawberry contains a PstI site.  To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI.  Digest the upstream sequence at EcoRI and Spe1.
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===Emission Spectrum===
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[[Image:MStrawberry_graph.jpg|600px|center]]
  
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Emission spectrum generated by mStrawberry reporter with 2*SE intervals shown for each reading.  Spectrum is the average percent increase in mStrawberry emission over that of DH5 alpha cells.  Cells were excited at 500nm.  Peak emission is at 596nm, as expected.<sup>1</sup>
  
 
===Source===
 
===Source===
  
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The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University.
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mStrawberry was developed by Roger Tsien.<sup>1</sup>
  
 
===References===
 
===References===
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1.  Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572

Latest revision as of 03:26, 12 March 2011

mStrawberry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mStrawberry contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and Spe1.

Emission Spectrum

MStrawberry graph.jpg

Emission spectrum generated by mStrawberry reporter with 2*SE intervals shown for each reading. Spectrum is the average percent increase in mStrawberry emission over that of DH5 alpha cells. Cells were excited at 500nm. Peak emission is at 596nm, as expected.1

Source

mStrawberry was developed by Roger Tsien.1

References

1. Shaner, N. C., Campbell, R.E., Steinbach, P.A., Giepmans, Ben N.G., Palmer, A.E., & Tsien, R.Y. (2004). Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology 22 (12), 1567 - 1572