Difference between revisions of "Part:BBa K325239"
 
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{{:Templates/Cambridge-fireflycolour|colour=red|substitution=Gly326Ser}}
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<partinfo>BBa_K325239 short</partinfo>
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{{:Templates/Cambridge-fireflycolour|colour=red|substitution=Gly326Ser|id=239}}
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==Annotated Sequence==
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<partinfo>BBa_K325239 SequenceAndFeatures</partinfo>

Latest revision as of 03:22, 28 October 2010

Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)

Input: L-Arabinose
Output: Light (red)

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     Add Data       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a Gly326Ser mutation was used to create a red bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325239 against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

Annotated Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
    Illegal SpeI site found at 3044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3044
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334