Difference between revisions of "Part:BBa K318500"
(→Characterization) |
|||
| (3 intermediate revisions by one other user not shown) | |||
| Line 7: | Line 7: | ||
RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. | RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. | ||
See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA]] | See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA]] | ||
| + | |||
| + | aaaaaaaaaaaaaaaaaaa | ||
===Characterization=== | ===Characterization=== | ||
| − | Download Zip file | + | Download Zip file [[media:BBa_K318500_pSB1C3.zip]] |
| + | |||
| + | <br> | ||
| + | <center> | ||
| + | [[image:UW_L_Fucose.jpg|400px]] | ||
| + | |||
| + | '''Figure 1:''' L-Fucose content of plasmids K318500, K318501, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Colonic acid can be estimated by measuring L-fucose content. | ||
| − | + | <br> | |
| − | [[ | + | [[image:UW_cell_survivability.jpg|400px]] |
| + | '''Figure 2:''' Survivability of plasmids K318500, K318501, K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We saw around a 10x increase in survivability with RcsB being over produced at a pH=4. | ||
| + | </center> | ||
| + | <br> | ||
| + | For more detail on testing, please see our results page on our <html><a href="http://2010.igem.org/Team:Wisconsin-Madison">Team Wiki</a></html> for protocols, background information, and conclusions. | ||
| + | <br> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K318502 SequenceAndFeatures</partinfo> |
Latest revision as of 13:27, 6 October 2021
lacI pL + RBS + RcsA + TT
Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).
Usage and Biology
RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA
aaaaaaaaaaaaaaaaaaa
Characterization
Download Zip file media:BBa_K318500_pSB1C3.zip
Figure 1: L-Fucose content of plasmids K318500, K318501, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Colonic acid can be estimated by measuring L-fucose content.
Figure 2: Survivability of plasmids K318500, K318501, K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We saw around a 10x increase in survivability with RcsB being over produced at a pH=4.
For more detail on testing, please see our results page on our Team Wiki for protocols, background information, and conclusions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]