Difference between revisions of "Part:BBa K391006:Design"

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===Design Notes===
 
===Design Notes===
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We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on psb1C3. One out of two sets of primers included a histidine tag, which would be used to isolate DspB after it is expressed.
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We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (<i>Eco</i>RI, <i>Xba</i>I, <i>Spe</i>I, <i>Pst</i>I) on each primer in order to put it on psb1C3. One out of two sets of primers included a histidine tag, which would be used to isolate DspB after it is expressed.
  
 
===Source===
 
===Source===

Latest revision as of 14:03, 26 September 2012

DspB - carbohydrate digesting protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 967
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed forward and reverse primers with the appropriate standard BioBrick restriction sites (EcoRI, XbaI, SpeI, PstI) on each primer in order to put it on psb1C3. One out of two sets of primers included a histidine tag, which would be used to isolate DspB after it is expressed.

Source

The gene that encodes our protein, DspB, stems from the bacteria Actinobacillus actinomycetemcomitans.

References