Difference between revisions of "Part:BBa K498000:Design"

 
(→‎Source)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K498000 short</partinfo>
 
<partinfo>BBa_K498000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
none
+
The IvyTech_South-Bend Team created this hybrid part to grant the AHL - detector device T9002 and enzymatic/cholorimetric read out to (hopefully) to enable the design of a simplified (in the field) detection system (no fluorescence excitation required).  The inclusion of the beta-gal mediated conversion of the IPTG substrate to a yellow color should serve this purpose.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 16: Line 13:
  
  
−
===References===
+
BBa_T9002 (GFP Producer Controlled by 3OC6HSL Receiver Device) and BBa_I73200 (Lac Z)are Registry Parts that were created by Anna Labno (Haseloff lab) and Zhan Jian  Group: iGEM07_USTC respectively.

Latest revision as of 03:56, 28 October 2010

T9002 - LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1956
    Illegal BamHI site found at 1965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1732
    Illegal BsaI.rc site found at 2902


Design Notes

The IvyTech_South-Bend Team created this hybrid part to grant the AHL - detector device T9002 and enzymatic/cholorimetric read out to (hopefully) to enable the design of a simplified (in the field) detection system (no fluorescence excitation required). The inclusion of the beta-gal mediated conversion of the IPTG substrate to a yellow color should serve this purpose.

Source

Registry of Standard Parts


BBa_T9002 (GFP Producer Controlled by 3OC6HSL Receiver Device) and BBa_I73200 (Lac Z)are Registry Parts that were created by Anna Labno (Haseloff lab) and Zhan Jian Group: iGEM07_USTC respectively.