Difference between revisions of "Part:BBa K376004:Design"

 
(→‎References)
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K376004 short</partinfo>
 
<partinfo>BBa_K376004 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
N/A
 
−
 
  
 +
This protein was codon optimized for E. coli by DNA 2.0 and the the RBS was optimized using the Salis Lab RBS calculator http://salis.psu.edu/RBS_Calculator.shtml
  
 
===Source===
 
===Source===
  
−
N/A
+
Protein sequence was taken from:
 +
 
 +
Tielker D, Eichhof I, Jaeger KE, Ernst JF.  Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae.  Eukaryot Cell. 2009 (6):913-915
  
 
===References===
 
===References===
 +
 +
Tielker D, Eichhof I, Jaeger KE, Ernst JF.  2009.  Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae.  Eukaryot Cell. 2009 (6):913-915
 +
 +
Salis HM, Mirsky EA, Voigt CA.  2009.  Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol. 2009 Oct;27(10):946-50

Latest revision as of 21:31, 6 November 2010

Anaerobic Fluorescent Protein with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein was codon optimized for E. coli by DNA 2.0 and the the RBS was optimized using the Salis Lab RBS calculator http://salis.psu.edu/RBS_Calculator.shtml

Source

Protein sequence was taken from:

Tielker D, Eichhof I, Jaeger KE, Ernst JF. Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell. 2009 (6):913-915

References

Tielker D, Eichhof I, Jaeger KE, Ernst JF. 2009. Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell. 2009 (6):913-915

Salis HM, Mirsky EA, Voigt CA. 2009. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol. 2009 Oct;27(10):946-50