Difference between revisions of "Part:BBa K305007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The sequence was retrieved from genomic ''Bacillus subtilis'' DNA using the following primers: | + | The sequence was retrieved from genomic ''Bacillus subtilis'' DNA using PCR with the following primers: |
Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG | Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG | ||
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===Source=== | ===Source=== | ||
| − | + | [http://dbtbs.hgc.jp/COG/prom/srfAA-srfAB-comS-srfAC-srfAD.html dbts] | |
| + | |||
| + | [http://subtiwiki.uni-goettingen.de/wiki/index.php/SrfAA SubtiWiki SrfAA] | ||
===References=== | ===References=== | ||
Latest revision as of 22:06, 7 November 2010
srfA promoter, short variant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was retrieved from genomic Bacillus subtilis DNA using PCR with the following primers:
Srfa-short-for: ATCAGAATTCGCGGCCGCTTCTAGAGGATTGAACGCAGCAGTTTG
Srfa-short-rev: ACTACTGCAGCGGCCGCTACTAGTACCCACCCTAATAAGAAACC
Source
[http://dbtbs.hgc.jp/COG/prom/srfAA-srfAB-comS-srfAC-srfAD.html dbts]
[http://subtiwiki.uni-goettingen.de/wiki/index.php/SrfAA SubtiWiki SrfAA]