Difference between revisions of "Part:BBa K338004"
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This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains ''phaB1'' and ''phaC1'': <partinfo>BBa_K156013</partinfo> & <partinfo>BBa_K156014</partinfo>. It was designed to be ligated downstream of part <partinfo>BBa_K338003</partinfo>. | This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: ''phaA'', ''phaB1'', ''phaC1''. This half contains ''phaB1'' and ''phaC1'': <partinfo>BBa_K156013</partinfo> & <partinfo>BBa_K156014</partinfo>. It was designed to be ligated downstream of part <partinfo>BBa_K338003</partinfo>. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here--> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Although not a finished product, it was tested in DH5α cells grown in 5mM glucose for 48 hours (5mL LB culture) at 37°C. The cells were then imaged at 100x, producing the following figures: | Although not a finished product, it was tested in DH5α cells grown in 5mM glucose for 48 hours (5mL LB culture) at 37°C. The cells were then imaged at 100x, producing the following figures: | ||
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[[Image:Caltech10Plastic2.jpg]] | [[Image:Caltech10Plastic2.jpg]] | ||
| − | The small inclusion bodies suggest the production of an unknown, but possibly plastic-related, substance. | + | The small inclusion bodies suggest the production of an unknown, but possibly plastic-related, substance. A similar construct, pSYL105, caused the formation of large inclusion bodies in cells that produced plastic, lending credence to this possibility. [1] |
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Latest revision as of 12:12, 26 October 2010
PHA Synthase Composite, Part 2/2
This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains phaB1 and phaC1: BBa_K156013 & BBa_K156014. It was designed to be ligated downstream of part BBa_K338003.
Usage and Biology
Although not a finished product, it was tested in DH5α cells grown in 5mM glucose for 48 hours (5mL LB culture) at 37°C. The cells were then imaged at 100x, producing the following figures:
The small inclusion bodies suggest the production of an unknown, but possibly plastic-related, substance. A similar construct, pSYL105, caused the formation of large inclusion bodies in cells that produced plastic, lending credence to this possibility. [1]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1348
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 697
Illegal AgeI site found at 1047 - 1000COMPATIBLE WITH RFC[1000]