Difference between revisions of "Part:BBa K337017"
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<partinfo>BBa_K337017 short</partinfo> | <partinfo>BBa_K337017 short</partinfo> | ||
| − | Bidirectional CMV promoter driving the GOI on (+) and/or (-) strand. | + | Bidirectional CMV promoter driving the GOI on (+) and/or (-) strand (BB-2, RFC12). |
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<partinfo>BBa_K337017 parameters</partinfo> | <partinfo>BBa_K337017 parameters</partinfo> | ||
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| + | === Characterization of the bidirectional CMV promoter === | ||
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| + | The bidirectional CMV promoter is a new standard part out of our [http://2010.igem.org/Team:Heidelberg/Project/miMeasure miMeasure construct]. | ||
| + | The promoter was used to express two reporter genes with a comparable transcription rate. Referring to the figures below, this is the case. | ||
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| + | [[Image:HeLa nobinding imageOverview.png|thumb|center|600px| Left panel: EGFP channel. Middle panel: EBFP channel. Right panel: merge of both the others.]] | ||
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| + | [[Image:GFPvsBFPHeLa nobinding miM.png|thumb|center|400px| EBFP intensities against EGFP intensities. Correlation coefficient is given.]] | ||
Latest revision as of 02:06, 28 October 2010
bidirectional CMVmin Promoter
Bidirectional CMV promoter driving the GOI on (+) and/or (-) strand (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization of the bidirectional CMV promoter
The bidirectional CMV promoter is a new standard part out of our [http://2010.igem.org/Team:Heidelberg/Project/miMeasure miMeasure construct]. The promoter was used to express two reporter genes with a comparable transcription rate. Referring to the figures below, this is the case.
