Difference between revisions of "Part:BBa K302030:Design"
 
Line 7: Line 7:
 
*Part will only work with the addition of 10% sucrose to the media.
 
*Part will only work with the addition of 10% sucrose to the media.
 
*Should be integrated at the ''sacB'' locus to prevent native Levansucrose glue production.
 
*Should be integrated at the ''sacB'' locus to prevent native Levansucrose glue production.
 +
*Sequence selected based on the work of Steinmetz et al. and Meng and Futterer in the papers below
  
 
===Source===
 
===Source===
Line 13: Line 14:
  
 
===References===
 
===References===
 +
 +
Steinmetz, M., Le Coq, D., Aymerich, S., Gonzy-Treboul, G. &
 +
Gay, P. (1985). The DNA sequence of the gene for the secreted
 +
''Bacillus subtilis'' enzyme levansucrase and its genetic control sites. ''Mol Gen Genet'' 200, 220–228.
 +
 +
Meng, G. & Futterer, K. (2003). Structural framework of fructosyl
 +
transfer in ''Bacillus subtilis'' levansucrase. ''Nat Struct Biol'' 10, 935–941.

Latest revision as of 01:38, 26 October 2010

IPTG-inducible Levansucrose glue


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

  • Part will only work with the addition of 10% sucrose to the media.
  • Should be integrated at the sacB locus to prevent native Levansucrose glue production.
  • Sequence selected based on the work of Steinmetz et al. and Meng and Futterer in the papers below

Source

Bacillus Subtilis 168 strain

References

Steinmetz, M., Le Coq, D., Aymerich, S., Gonzy-Treboul, G. & Gay, P. (1985). The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites. Mol Gen Genet 200, 220–228.

Meng, G. & Futterer, K. (2003). Structural framework of fructosyl transfer in Bacillus subtilis levansucrase. Nat Struct Biol 10, 935–941.