Difference between revisions of "Part:BBa K392039"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K392039 short</partinfo>
 
<partinfo>BBa_K392039 short</partinfo>
  
 
Device containing C. fermi endoglucanase CenA with E. coli PelB export tag attached upstream to enable secretion of protein.
 
Device containing C. fermi endoglucanase CenA with E. coli PelB export tag attached upstream to enable secretion of protein.
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See experience page for experimental data.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:36, 8 November 2010

endoglucanase secretion testing construct for E. coli

Device containing C. fermi endoglucanase CenA with E. coli PelB export tag attached upstream to enable secretion of protein.

See experience page for experimental data.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 331
    Illegal NotI site found at 1475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1388
    Illegal BamHI site found at 524
    Illegal XhoI site found at 886
    Illegal XhoI site found at 1135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 666
    Illegal NgoMIV site found at 1591
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 570