Difference between revisions of "Part:BBa K302034"
 
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<partinfo>BBa_K302034 short</partinfo>
 
<partinfo>BBa_K302034 short</partinfo>
  
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Encodes a stable non-specific ribonuclease toxin (''mazF'') and its inhibitory antitoxin (''mazE'') in ''Bacillus Subtilis''. These genes are use in ''Bacillus Subtilis'' to provide a toxin-antitoxin in various stressful conditions. When transcribtion of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.
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Encodes a stable non-specific ribonuclease toxin (''mazF'') and its inhibitory antitoxin (''mazE'') in ''Bacillus Subtilis''. These genes are used in ''Bacillus Subtilis'' to provide a toxin-antitoxin kill switch in various stressful conditions. When expression of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''mazF'', killing the cell.
  
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===Other Uses===
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===Usage and Biology===
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This toxin-antitoxin system has been improved and adapted for different uses twice so far. The most effective use for a long term bioengineering purpose can be seen in [[part:BBa_K3588014]] or at https://2020.igem.org/Team:CLS_CLSG_UK/Design
  
 
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Latest revision as of 02:12, 27 October 2020

mazEF toxin-antitoxin cluster

Encodes a stable non-specific ribonuclease toxin (mazF) and its inhibitory antitoxin (mazE) in Bacillus Subtilis. These genes are used in Bacillus Subtilis to provide a toxin-antitoxin kill switch in various stressful conditions. When expression of both genes is turned off (as both are under contol of same promoter) mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.

Other Uses

This toxin-antitoxin system has been improved and adapted for different uses twice so far. The most effective use for a long term bioengineering purpose can be seen in part:BBa_K3588014 or at https://2020.igem.org/Team:CLS_CLSG_UK/Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]