Difference between revisions of "Part:BBa K339007:Experience"
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===Applications of BBa_K339007=== | ===Applications of BBa_K339007=== | ||
| + | We are Team Tianjin of iGEM 2016, we aim at biodegrading PET by enzymes and we want to introduce the inclusion body regulation system in our project. This part can be used to report the generation of inclusion body in E.coli, and what we did is that we change the mRFP gene to the ddpX gene, which can cause cell lysis when overexpress. Then the E.coli will break down after too many inclusion bodies are in the periplasm so that we can directly purify the protein without the complicated step of breaking cells. | ||
===User Reviews=== | ===User Reviews=== | ||
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<partinfo>BBa_K339007 AddReview number</partinfo> | <partinfo>BBa_K339007 AddReview number</partinfo> | ||
| − | <I> | + | <I>Tianjin_iGEM 2016</I> |
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| − | + | This part was firstly verified by us and used as the reporting system of our PETase gene. Then we improve the application by change the mRFP gene to the ddpX gene which can cause cell lysis and offer a new and easy way to purify the protein. It's useful and we think this is truely a good part. | |
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Latest revision as of 11:38, 10 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K339007
We are Team Tianjin of iGEM 2016, we aim at biodegrading PET by enzymes and we want to introduce the inclusion body regulation system in our project. This part can be used to report the generation of inclusion body in E.coli, and what we did is that we change the mRFP gene to the ddpX gene, which can cause cell lysis when overexpress. Then the E.coli will break down after too many inclusion bodies are in the periplasm so that we can directly purify the protein without the complicated step of breaking cells.
User Reviews
UNIQ4e028197d4d55fae-partinfo-00000000-QINU UNIQ4e028197d4d55fae-partinfo-00000001-QINU