Difference between revisions of "Part:BBa K364332:Design"
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===Design Notes=== | ===Design Notes=== | ||
Compatible with RFC-10 and RFC-25. | Compatible with RFC-10 and RFC-25. | ||
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===Source=== | ===Source=== | ||
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Artificial and human PXR LBD. | Artificial and human PXR LBD. | ||
Latest revision as of 23:46, 25 October 2010
TRE-Gal4-PXR-PolyA in PSB1A3 (expression vector)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 912
Illegal BglII site found at 1023
Illegal BamHI site found at 1446
Illegal XhoI site found at 547 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 466
Design Notes
Compatible with RFC-10 and RFC-25.
Source
Artificial and human PXR LBD.
References
Guhaniyogi J, Brewer G. Regulation of mRNA stability in mammalian cells. Gene. 2001 Mar 7;265(1-2):11-23.
D R Gallie. The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency. Genes Dev. 1991 5: 2108-2116
1.Lehmann JM, McKee DD, Watson MA, Willson TM, Moore JT, Kliewer SA (September 1998). "The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions". J. Clin. Invest. 102 (5): 1016–23. doi:10.1172/JCI3703.
Ana Traven, Branka Jelicic & Mary Sopta. "Yeast Gal4: a transcriptional paradigm revisited". EMBO reports (2006) 7, 496 - 499.