Difference between revisions of "Part:BBa K321003:Design"

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===Design Notes===
 
===Design Notes===
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A pre-existing PstI site was removed to meet the BioBrick standard.
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A pre-existing PstI site was removed to meet the BioBrick standard.  A PCR product encoding the sequence was flanked with an XbaI site at the 5' and an SpeI site at the 3' for ligation with XbaI, SpeI double-digested pSB1C3 vector.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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 +
'''Cellular ligands of activating'''
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'''NK receptors'''
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 +
Cristina Bottino1, Roberta Castriconi, Lorenzo Moretta and Alessandro Moretta
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 +
Trends Immunol. 2005 Apr;26(4):221-6.
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 +
http://www.ncbi.nlm.nih.gov/pubmed/15797513
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 +
'''Dissecting natural killer cell activation pathways through analysis of genetic mutations in human and mouse.'''
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 +
Tassi I, Klesney-Tait J, Colonna M.
 +
 +
Immunol Rev. 2006 Dec;214:92-105.
 +
 +
http://www.ncbi.nlm.nih.gov/pubmed/17100878
 +
 +
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'''Natural killer cell activation in mice and men: different triggers for similar weapons?'''
 +
 +
Colucci F, Di Santo JP, Leibson PJ.
 +
 +
Nat Immunol. 2002 Sep;3(9):807-13.
 +
 +
http://www.ncbi.nlm.nih.gov/pubmed/12205470

Latest revision as of 02:23, 26 October 2010

intracellular chain of CD3 zeta


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 274


Design Notes

A pre-existing PstI site was removed to meet the BioBrick standard. A PCR product encoding the sequence was flanked with an XbaI site at the 5' and an SpeI site at the 3' for ligation with XbaI, SpeI double-digested pSB1C3 vector.

Source

Cloned from the human genome.

References

Cellular ligands of activating NK receptors

Cristina Bottino1, Roberta Castriconi, Lorenzo Moretta and Alessandro Moretta

Trends Immunol. 2005 Apr;26(4):221-6.

http://www.ncbi.nlm.nih.gov/pubmed/15797513

Dissecting natural killer cell activation pathways through analysis of genetic mutations in human and mouse.

Tassi I, Klesney-Tait J, Colonna M.

Immunol Rev. 2006 Dec;214:92-105.

http://www.ncbi.nlm.nih.gov/pubmed/17100878


Natural killer cell activation in mice and men: different triggers for similar weapons?

Colucci F, Di Santo JP, Leibson PJ.

Nat Immunol. 2002 Sep;3(9):807-13.

http://www.ncbi.nlm.nih.gov/pubmed/12205470