Difference between revisions of "Part:BBa K320004"

 
(6 intermediate revisions by 2 users not shown)
Line 3: Line 3:
  
  

 This BioBrick is constitued by an origin of replication for Asaia and E. coli (<partinfo> BBa_K320000 </partinfo>) and a resistance to Kanamycin (<partinfo> BBa_P1003 </partinfo>). It was initially designed to be inserted into (<partinfo>BBa_I51020 </partinfo>.  
+

 This BioBrick consists of an origin of replication for Asaia and E. coli (<partinfo>BBa_K320000</partinfo>) and a resistance to Kanamycin (<partinfo>BBa_P1003</partinfo>) . It was initially designed to be inserted into (<partinfo>BBa_I51020 </partinfo>).  
  
<!-- Add more about the biology of this part here
+
We developed this BioBricks as a tool for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia'']
 +
 
 +
<!-- Add more about the biology of this part here  
 
===Usage and Biology===
 
===Usage and Biology===
  
<!-- -->
+
-->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K320004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K320004 SequenceAndFeatures</partinfo>
  
  
<!-- Uncomment this to enable Functional Parameter display  
+
<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K320004 parameters</partinfo>
 
<partinfo>BBa_K320004 parameters</partinfo>
<!-- -->
+
-->

Latest revision as of 16:23, 27 October 2010

Origin for Asaia and resistance to Kanamycin



 This BioBrick consists of an origin of replication for Asaia and E. coli (BBa_K320000) and a resistance to Kanamycin (BBa_P1003) . It was initially designed to be inserted into (BBa_I51020).

We developed this BioBricks as a tool for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4