Difference between revisions of "Part:BBa K364326:Experience"
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===Ligand===
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Ecdysteroids
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Ecdysteroids  are  insect  moulting  and  sex  hormones  which  include  ecdysone  and
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its  homologues  such  as  20-hydroxyecdysone.  Ecdysteroids  also  occur  in  other
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invertebrates where they can play a different role. They also appear in many plants
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mostly  as  a  protection  agents  (toxins  or  antifeedants)  against  herbivore  insects.
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These  phytoecdysteroids  have  medicinal  value  and  are  part  of  herbal  adaptogenic
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remedies like cordyceps.
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===Physiological functions of EcR===
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Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, as evidenced by chromosomal puffing at over a hundred sites. Ultimately the activation cascade causes physiological changes that result in ecdysis (moulting).
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===Potential applications of GAL4-EcR LBD===
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By the addition of lipid ligands(ecdysteroids), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
  
 
===Applications of BBa_K364326===
 
===Applications of BBa_K364326===
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[[Image:Team Debrecen WB pCDNA-Gal4-EcR.jpg|800px|thumb|center|Picture of Western blot: Gal4-EcR composite construct is expressed after transferring it into pCDNA3.1]]
 
[[Image:Team Debrecen WB pCDNA-Gal4-EcR.jpg|800px|thumb|center|Picture of Western blot: Gal4-EcR composite construct is expressed after transferring it into pCDNA3.1]]
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[[Image:Team Debrecen WB pCDNA-Gal4-EcR2.jpg|800px|thumb|center|Picture of Western blot: Gal4-EcR composite construct is highly expressed after transferring it into pCDNA3.1]]
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[[Image:PXR,_Ecr2.jpg|450px|thumb|center|Test of pCDNA/pSB1A3-GAL4-PXR/EcR-LBD activity]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 07:44, 27 October 2010


Ligand

Ecdysteroids

Ecdysteroids are insect moulting and sex hormones which include ecdysone and its homologues such as 20-hydroxyecdysone. Ecdysteroids also occur in other invertebrates where they can play a different role. They also appear in many plants mostly as a protection agents (toxins or antifeedants) against herbivore insects. These phytoecdysteroids have medicinal value and are part of herbal adaptogenic remedies like cordyceps.

Physiological functions of EcR

Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, as evidenced by chromosomal puffing at over a hundred sites. Ultimately the activation cascade causes physiological changes that result in ecdysis (moulting).

Potential applications of GAL4-EcR LBD

By the addition of lipid ligands(ecdysteroids), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364326

Picture of gel electrophoresis: Gal4-EcR in pCDNA3.1 resulting an insert of 1142 bp.
Picture of Western blot: Gal4-EcR composite construct is expressed after transferring it into pCDNA3.1
Picture of Western blot: Gal4-EcR composite construct is highly expressed after transferring it into pCDNA3.1
Test of pCDNA/pSB1A3-GAL4-PXR/EcR-LBD activity

User Reviews

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