Difference between revisions of "Part:BBa K395302"
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<partinfo>BBa_K395302 short</partinfo> | <partinfo>BBa_K395302 short</partinfo> | ||
− | This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of P''OmpC(WT)''BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. ''OmpC( | + | This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of P''OmpC(WT)''BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. ''OmpC(CB)'' promoter consists of binding site 1 and additional 10 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series] |
+ | [[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 hours. We characterized this promoter by attaching GFP(E0240) to the downstream and mearsuring its transcriptional activity through the GFP expression. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010.]] | ||
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Latest revision as of 00:21, 28 October 2010
PompC(CB) Promoter regulated by phosphorylated OmpR
This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(CB) promoter consists of binding site 1 and additional 10 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 27
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]