Difference between revisions of "Part:BBa K422014:Design"
 
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<partinfo>BBa_K422014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K422014 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. CyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
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BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. mCyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
  
 
===Source===
 
===Source===
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===References===
 
===References===
 
http://2010.igem.org/Team:ETHZ_Basel
 
http://2010.igem.org/Team:ETHZ_Basel
−
Ohashi, Galiacy, Briscoe and Harold: An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Science. 2007; 16.
+
 
 +
Ohashi, Galiacy, Briscoe and Erickson: An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Science. 2007; 16.
 +
 
 +
Nguyen and Daugherty: Evolutionary optimization of fluorescent proteins for intracellular FRET. Nature Biotechnology. 2005; 23.

Latest revision as of 10:28, 27 October 2010

mCyPet (AarI A-part)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 733
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 733
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 733
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 733
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. mCyPet-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Source

Codon optimized.

References

http://2010.igem.org/Team:ETHZ_Basel

Ohashi, Galiacy, Briscoe and Erickson: An experimental study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Science. 2007; 16.

Nguyen and Daugherty: Evolutionary optimization of fluorescent proteins for intracellular FRET. Nature Biotechnology. 2005; 23.