Difference between revisions of "Part:BBa K422010"

Latest revision as of 11:33, 25 October 2010

trig (AarI A-part)

Biological Background

The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome.

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. Trig-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 364
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 364
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 364
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 364
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 88

@@ Line 3: / Line 3: @@
 ===Biological Background===
-The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome [1].
+The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome.
 ===Cloning strategy===
-BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. '''CheB-A''' is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
+BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. '''Trig-A''' is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].
-===References===
-[1] Hesterkamp, Deuerling and Bukau: The Amino-terminal 118 amino acids of Escherichia coli Trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. The Journal of Biologcial Chemistry. 1997; 272:35.