Difference between revisions of "Part:BBa K364320:Experience"
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+ | ===Physiological effects of NHR-8=== | ||
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+ | NHR-8 is required for gut function and may respond to the food signal. | ||
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+ | The nhr-8 gene mutants have abnormally low resistance to the toxins colchicine and chloroquine.This resembles the regulation of protective metabolic enzymes by xenobiotic-sensing nuclear receptors. | ||
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+ | NHR-8 may thus mediate a subset of the resistance traits promoted by PGP-3. | ||
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+ | ===Possible applications of GAL4-NHR-8=== | ||
+ | By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase). | ||
===Applications of BBa_K364320=== | ===Applications of BBa_K364320=== | ||
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+ | [[Image:nhr8pcdna.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-NHR8 in pCDNA3.1 resulting an insert of 953 bp.]] | ||
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+ | [[Image:Team Debrecen WB pCDNA-Gal4-NHR8.jpg|800px|thumb|center|Picture of Western blot: Gal4-NHR8 composite construct is highly expressed after transferring it into pCDNA3.1]] | ||
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+ | [[Image:nhr64.jpg|center|600px|thumb|Activation of Gal-NHR8 and Gal-NHR64 to oil sand extractions.]] | ||
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+ | [[Image:nhr8.jpg|center|300px|thumb|Activation of NHR8 to Blesse bleu cheese extract.]] | ||
===User Reviews=== | ===User Reviews=== |
Latest revision as of 07:45, 21 September 2011
Physiological effects of NHR-8
NHR-8 is required for gut function and may respond to the food signal.
The nhr-8 gene mutants have abnormally low resistance to the toxins colchicine and chloroquine.This resembles the regulation of protective metabolic enzymes by xenobiotic-sensing nuclear receptors.
NHR-8 may thus mediate a subset of the resistance traits promoted by PGP-3.
Possible applications of GAL4-NHR-8
By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
Applications of BBa_K364320
User Reviews
UNIQ45a1bbad348c2b9a-partinfo-00000000-QINU UNIQ45a1bbad348c2b9a-partinfo-00000001-QINU